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. 2002 Jan 21;195(2):181–188. doi: 10.1084/jem.20011453

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — FACS^® analysis of mature B cells in anti-DNA H chain sd-tg mice. (A) Splenic and bone marrow cells were isolated from 3H9H, 3H9H/56R, and 3H9H/56R/76R sd-tg mice. Cells were stained with antibodies against B220, CD43, IgM, and IgD. All analyses were performed on the lymphocyte-gated population. IgM⁺IgD⁺ cells were plotted from the B220⁺CD43⁻-gated population. (B) Recognition of transgenic H chain–bearing cells by the anti-idiotypic Ab, 1.209, which recognizes the 3H9 H chain in combination with most L-chain. Cells were double-stained with anti-B220 and 1.209. Data were plotted and percentages calculated from the lymphocyte-gated population. These results are representative of four independent experiments.