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. 2002 Jan 21;195(2):181–188. doi: 10.1084/jem.20011453

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — κ and λ expression in spleen cells of mice with H chain of different DNA binding affinities. (A) Spleen cells from BALB/c, 3H9, 3H9H/56R, 3H9H/56R/76R sd-tg mice were stained with anti-κ and -λ. Cells were gated on a lymphoid gate, and percentages of κ⁺, λ⁺, and κ/λ⁺ are indicated. (B) 3H9H/56R mice have a large population of κ/λ double-positive B cells. Spleen cells from 3H9H/56R and the nontransgenic littermate were stained with anti-B220, κ, and λ. Dead cells were excluded by propidium iodide staining. Percentages of κ⁺, λ⁺, and κ/λ⁺ cells in a B220⁺ gate are indicated. Representative of experiments using three different mice of each kind. (C) Surface IgM expression of κ/λ double-positive B cells are higher than most of the κ single-positive B cells in 3H9H/56R mice. Histograms of IgM expression are shown for the κ/λ double-positive (R4 and thin line) and κ single-positive (R5 and bold line) population.

Figure 2. — κ and λ expression in spleen cells of mice with H chain of different DNA binding affinities. (A) Spleen cells from BALB/c, 3H9, 3H9H/56R, 3H9H/56R/76R sd-tg mice were stained with anti-κ and -λ. Cells were gated on a lymphoid gate, and percentages of κ⁺, λ⁺, and κ/λ⁺ are indicated. (B) 3H9H/56R mice have a large population of κ/λ double-positive B cells. Spleen cells from 3H9H/56R and the nontransgenic littermate were stained with anti-B220, κ, and λ. Dead cells were excluded by propidium iodide staining. Percentages of κ⁺, λ⁺, and κ/λ⁺ cells in a B220⁺ gate are indicated. Representative of experiments using three different mice of each kind. (C) Surface IgM expression of κ/λ double-positive B cells are higher than most of the κ single-positive B cells in 3H9H/56R mice. Histograms of IgM expression are shown for the κ/λ double-positive (R4 and thin line) and κ single-positive (R5 and bold line) population.