Figure 4.
Donor-derived cells in the draining LNs are bone marrow derived. The normal cornea exhibited strong expression of keratin 12 (K-12) (A). However, examination of the draining LNs after corneal transplantation did not exhibit any sign of K-12+ cells at any time point studied (B). Numerous CD45+ (red) cells were seen in the LNs and double staining for donor Iab (green) exhibited colocalization (yellow) mostly in the parafollicular areas (arrows) of the draining LNs (C). No purely green cell was observed, confirming that all donor MHC class II+ cells are bone marrow derived (CD45+). Original magnifications 400× (A), 160× (B), and 400× (C).