Figure 6.
Analysis of gut populations in different mutant mice (A) TCR rearrangements in Lin− cells from CD3-εΔ5/Δ5 mice were studied as described in Fig. 5 B. The total amount of DNA (determined by amplification of the germline Cβ gene) is shown. Please note that samples from CD3-εΔ5/Δ5 mice contained the same amount of DNA and were amplified simultaneously with those from normal mice, as shown in Fig. 5 B. Results are from one of three independent sortings and show the experiment in which we detected the largest amounts of rearranged receptors. In one of these sortings, we could not detect TCR-γ rearrangements in CD3-εΔ5/Δ5–deficient cells. (B) TCR-β expression in CD8αα T-IEL from pre-Tα+/+ (left) and pre-Tα−/− mice (right), in one out of four experiments, with the same results. (C) Effects of TCR-α and -δ deficiencies in Lin− IEL. Results show the CD8/Thy1 phenotype of Lin− IEL in TCR-α– (left) and TCR-δ– (right) deficient mice in one out of eight (left), and two (right), experiments with similar results. In both mice, Thy1-SP-IEL were IL-7R− CD25− (unpublished data). These mutations did not change the phenotype of CP cells.