Table II.
Gene Expression in Lin− Gut Cells
| Lin− CP
|
Lin− IEL
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Thy1−CD8− | Thy1-CP | Percent in CD3+ |
Thy1-SP | Percent in CD3+ |
DP | Percent in CD3+ |
DN | CD8-SP | |
| No. cells screeneda | 6,000 | 24,000 | 4,500 | 3,500 | 16,000 | 16,000 | |||
| CD3-εb | NEG | 6.3 (2.8) | 12.6 (5) | 15.5 (2) | 91 | 87 | |||
| Pre-Tαb | NEG | 0.14 (0.005) | 2.2 | 1.5 (1.6) | 12 | 0.14 (0.16) | 0.9 | NEGc | NEG |
| Rag-1b | NEG | 0.33 (0.1) | 5.2 | 0.02 (0.05) | 0.2 | 0.036 (0.007) | 0.2 | 0.006 (0.0013) | NEG |
a
Lin− gut cells were sorted at 500 cells/well and screened for CD3-ε, pre-Tα, and Rag-1 mRNA expression. NEG, not detected in all screened cells.
b
Cells were sorted in limiting dilution and the frequency of mRNA expressing cells were calculated according to the Poisson distribution (95% confidence limits are shown in brackets). Plating efficiency was evaluated by HPRT mRNA amplification.
c
Expression was too rare to allow frequency determination.