Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Feb 18;195(4):461–472. doi: 10.1084/jem.20011543

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Characterization of the multivesicular compartments induced after BCR stimulation. Ultrathin cryosections of IIA1.6 cells stimulated for 30 min were double immunogold-labeled with the rat monoclonal M5.114 antibody and a polyclonal anti-invariant chain antibody (A), a polyclonal anti-FITC antibody (B) or a rat monoclonal anti-Lamp1 antibody (D). The same cells were also single immunogold-labeled with a rabbit polyclonal anti-Igα antibody (C). In D, a biotinylated M5.114 antibody was used to avoid cross-reactions. In E and F, ultrathin cryosections of unstimulated IIA1.6 cells (E) or cells stimulated for 30 min (F) were double immunogold-labeled with the M5.114 antibody (PAG 10) and a biotinylated anti–H2-M antibody detected with an anti-biotin antibody (PAG 15). (A) Note the presence of the invariant chain (PAG 15) in the class II-rich multivesicular compartments (stars). (B) The FITC-coupled Igs (PAG 15) clearly accumulate in the class II molecule-containing multivesicular compartments (PAG 10). (C) The multivesicular MIICs also contain Ig α (PAG 10) (star). (D) Both Lamp1 (PAG 15) and MHC class II (PAG 10) are present in the multivesicular MIICs. (E) In unstimulated cells, most of the H2-M was present in compartments containing internal electron-dense membranes (Lys). (F) In cells stimulated for 30 min, H2-M was detected in characteristic class II molecule-containing multivesicular compartments (star). Bars, 200 nm.