ClustalW sequence alignment of PIGA orthologues from T. gondii, Plasmodium falciparum (gi23495183), Arabidopsis thaliana (gi18407913), Homo sapiens (gi219994), Mus musculus (gi1402592), Paramecium tetraurelia (gi8571458), and Saccharomyces cerevisiae (gi9755344). Regions of homology are boxed, with identity denoted by dark shading and conserved amino acid changes denoted by light shading. The predicted T. gondii PIGA transmembrane domain (amino acids 517 to 539) is denoted by a thick horizontal line.