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. 2002 Feb 18;195(4):391–399. doi: 10.1084/jem.20011974

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — The MAGE-3 antigenic peptide is presented by HLA-B40 molecules. Monkey COS-7 cells were transiently transfected with a MAGE-3 cDNA and each of the cDNAs coding for putative HLA-presenting molecules. Transfections were performed in microwells with 15,000 COS-7 cells, 50 ng of each cDNA and 1 μl of LIPOFECTAMINE. 1 d after transfection, 2,500 cells of CTL 52 were added to the transfected cells. TNF production was measured after overnight coculture by testing the toxicity of the supernatants for TNF-sensitive WEHI-164 clone 13 cells.