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. 2002 Feb 18;195(4):401–413. doi: 10.1084/jem.20011433

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Inter-strain variability of TSSA. (A) Genomic DNA from the indicated T. cruzi strains or cloned stocks was digested by ApaLI and hybridized under stringent conditions with the tssa probe. Size markers (in kpb) are indicated on the left. (B) Comparison of the deduced TSSA amino acid sequences from the indicated parasite isolates. Isolates were classified as T. cruzi I or II according to reference 5. Amino acid identities with the CL Brener sequence are shadowed. Hyphens were introduced for better alignment. (C) tssa and lineage typing of T. cruzi strains and cloned stocks. A fragment of the tssa gene was amplified from DNA samples of the indicated isolates, digested with PvuII and resolved by PAGE. From the same samples the rDNA and mini-exon intron were amplified and resolved in agarose gels. An example of the pattern observed for each lineage as determined in this work for tssa and already described for the rDNA and mini-exon (reference 5) is shown at left. The results for all the isolates are summarized in the table at right. ND, not determined.