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. 1999 May 17;189(10):1545–1554. doi: 10.1084/jem.189.10.1545

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Plasma samples from HIV-infected patients were obtained and immediately separated into 1-ml aliquots, which were analyzed by each of two methods with blinded results. In addition, a standard preparation of HIV RNA was obtained from the National Institute of Allergy and Infectious Diseases AIDS Research and Reference Reagent Program and analyzed by the UAB QC-RT-PCR procedure. Roche Monitor^® assay was performed as per manufacturer's instructions. Specimens with viral loads >5 × 10⁵/ml were analyzed after appropriate dilution. The UAB QC-RT-PCR procedure was performed as described in Materials and Methods.