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. 1999 May 17;189(10):1649–1658. doi: 10.1084/jem.189.10.1649

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Suppression of Syt II expression. Whole lysates derived from G418-resistant RBL clones (1.5 × 10⁶ cell equivalents), transfected with either the pcDNA3-Syt II antisense orientation (Syt II⁻, lanes 1–4) or with the empty pcDNA3 vector (control, lanes 5–7) were resolved by SDS-PAGE and immunoblotted as in Fig. 3.