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. 1999 May 17;189(10):1649–1658. doi: 10.1084/jem.189.10.1649

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Subcellular fractionation of control and Syt II–transfected RBL cell lysates. Fractions from a continuous sucrose gradient were collected from the top, and assayed for: A, Syt II immunoreactivity in control cells; B, Syt II immunoreactivity in Syt II⁺ cells; C, Syt I immunoreactivity in Syt I⁺ cells; D, pro-cathepsin D immunoreactivity; E, β-hexosaminidase activity (presented as OD read at 405 nm) (▪); histamine content (□) and LDH activity (•); (F) protein (•) and sucrose density (○). The data presented in panels E and F is the average of three sucrose gradients performed on control, Syt II⁺, and Syt I⁺ cells.