Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Jan 8;152(1):181–196. doi: 10.1083/jcb.152.1.181

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Src acts as an adaptor molecule mediating interactions between Cbl and Pyk2. (A) Association of Cbl with Pyk2 in presence of Src kinase mutants was analyzed by probing the blot of Cbl immunoprecipitates with Pyk2 antibodies (top). The blot was stripped and reprobed with Cbl antibodies. Total cell lysates (TCL) were Western blotted with Pyk2 and avian Src antibodies to check expression of transfected proteins. (B) 293-VnR cells were transfected with Pyk2, PKM, or empty vector (pBK). Cbl was immunoprecipitated from lysates of replated cells and analyzed for the presence of phosphotyrosine (top). (C) 293-VnR cells were transfected with Pyk2 mutants. The attachment-induced association of the mutant Pyk2 proteins with Cbl (top) was analyzed by blotting Cbl IPs with anti–Pyk2 antibody. (D) Cbl IPs from lysates of 293-VnR cells transfected with 10 μg of the indicated Src expression vector were blotted with an antiphosphotyrosine antibody to demonstrate the effects of these Src mutants of Cbl phosphorylation. Src was immunoprecipitated from the same lysates and the immune complexes were blotted for the presence of Cbl. (E) 293-VnR cells were transfected with a combination of 3 μg Pyk2 and 3 μg of the indicated Src expression vector. Cbl IPs were analyzed for the presence of Pyk2 (top). (Bottom) Expression of the Src mutants in total cell lysates (TCL).