Figure 3.

Aut2p is required for the GFPAut7p association to punctate, perivacuolar structures. The apg1Δ (NNY20) and aut2Δ (WPHYD2) strains were transformed with the pCuGFPAUT7 plasmid. The cells were grown to midlog phase in SMD medium. GFPAut7p expression was then induced with 50 μM CuSO₄ for 3 h. Cells were then labeled with FM 4-64, shifted to SD-N medium for 3 h, and examined as described in Fig. 2. In apg1Δ cells, GFPAut7p is recruited to punctate structures, whereas in the Aut7p processing–defective aut2Δ cells, GFPAut7p appears uniformly distributed in the cytoplasm.