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. 2001 Jan 8;152(1):111–126. doi: 10.1083/jcb.152.1.111

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The hypervariable regions of Rho GTPases are sufficient for differential membrane targeting which is independent of the length of the prenyl chain. MDCK cells were transiently transfected with the indicated constructs that included GFP extended at the COOH terminus with the hypervariable regions (last 20 amino acids) of pCdc42hs (a), bCdc42hs (b), Rac1 (c), and RhoA (d) or amino acids 73–193 of RhoA (e). (f) A doubly chimeric fusion protein consisting of GFP followed by amino acids 1–188 of TC10 and then amino acids 172–191 of pCdc42hs. (g and h) The last amino acid of the hypervariable regions of pCdc42hs (g) and Rac1 (h) were changed from L to M to switch modification from geranylgeranylation to farnesylation. Bars, 10 μm.