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. 2001 Jan 8;152(1):111–126. doi: 10.1083/jcb.152.1.111

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The role of palmitoylation on localization and RhoGDIα binding of Rho GTPases. COS-1 (a–e, i, and j) or MDCK (f–h) cells were transiently transfected with the indicated GFP-tagged GTPase in the absence (a, c, e, f, h, and i) or presence (b, d, g, and j) of 25 μM 2BP and imaged alive after 24 h as described in the legend to Fig. 1. (f–h) GFP-tagged RhoA in which the last four amino acids (CLVL) were switched to the last five amino acids of RhoB (CCKVL) was expressed without (f) or with (g) 2BP or coexpressed with excess (1:2 DNA ratio) RhoGDIα (h). Bars, 10 μm.