FIG. 6.

Ask10p phosphorylation requires Mkk1p/Mkk2p. (A, B, and C) Strains with the indicated genotypes containing ASK10-HA expression plasmid (pAK3) were harvested in mid-log phase (T = 0) or 30 min after treatment with H₂O₂ (0.5 mM). Extracts were prepared, and Ask10p-HA was visualized as described in Materials and Methods. The hyperphosphorylated form of Ask10p is indicated by the shaded “p.”