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. 2002 Apr 15;195(8):991–1001. doi: 10.1084/jem.20010809

Figure 1.

Figure 1.

Transfectants expressing various TCR-α with RT1β failed to respond to P18IIIB. (A) A representative FACS® profile for the expression of TCR and GFP in transfectants expressing RT1β and a TCR-α chain. TG40, the TCR-αβ–deficient T cell hybridoma cell line (a), was transfected with RT1β chain, and GFP before TCR-α transfection (b). Vα42H11+ TCR-α chains were randomly isolated from naive CD8+ cells from wild-type BALB/c mice, and these TCR-α genes were transfected into TCR-β–expressing TG40 cells by retrovirus-mediated gene transfer. The expression of TCR was monitored by the intensity of CD3ɛ-PE and 30–60% of the transfectant expressed the cell surface TCR complex (c). (B) IL-2 production of TCR-αβ transfectants upon stimulation with Ag/MHC and anti-TCR-β Ab. The transfectants were stimulated with irradiated splenocytes and P18IIIB (right) or immobilized anti–TCR-β Ab, H57 (left) for 24 h. The concentration of secreted IL-2 was measured by ELISA and the data are presented as the percentages of RT1-TCR-αβ-transfectant (RT1β).