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. 2002 Apr 15;195(8):991–1001. doi: 10.1084/jem.20010809

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Ag-specific cytotoxicity and proliferation of CD8⁺ T cells from RT1-TCR-α Tg and/or TCR-β Tg mice. (A) Ag-specific proliferation. Purified CD8⁺ spleen cells from RT1-TCR Tg or nonTg mice were cultured with irradiated spleen cells from BALB/c in the presence of 4 μM of P18IIIB (closed bar), P18MN (hatched bar), or no peptide (open bar). Cultures were pulsed with ³[H]-thymidine (TdR) for 8 h on day 3 of the culture and incorporated radioactivity was measured. (B) Ag-specific cytotoxicity. Spleen cells from unprimed RT1-TCR-αβ Tg (a), TCR-α Tg (b), TCR-β Tg (c), and normal mice (d) were stimulated in vitro with HIV IIIB gp160-transfected NIH 3T3 cells. After 5 d, the cytotoxic function of induced CTLs was assessed on NIH 3T3 target cells pulsed with P18IIIB (closed circles), P18MN (open circles), or no peptide (closed triangles) in a standard ⁵¹Cr-release assay.