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. 2002 Apr 15;195(8):1033–1041. doi: 10.1084/jem.20011607

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Analysis of the binding ability of mutant ICOS to B7-H2. Binding of wild-type and mutant ICOSIg to plate-bound B7-H2Ig (mIg) fusion protein was measured by ELISA. The concentrated supernatants (a–d) or purified proteins (e) from the cultures of transfected COS cells by indicated ICOS mutants were used. All determinations represent the mean of triplicate samples and differed from the mean by <10%. Shown is a representative of three independent experiments.