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. 2002 Apr 15;195(8):959–971. doi: 10.1084/jem.20011948

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Perinatal B7-blockade rescues VSAg-specific T cells in the thymus and in the periphery. BALB/c mice were treated with either anti-B7 or control IgG during the perinatal period. 4 wk after birth the thymi, spleens, and lymph nodes were harvested and stained with a cocktail of anti-CD4, anti-CD8, and anti-Vβ mAbs. (a) FACS^® profiles depict the Vβ subset distribution of gated CD4⁺CD8⁻ or CD4⁻CD8⁺ thymocytes. The numbers given in each panel are the percentage of cells expressing the given Vβs. Data from one representative thymus in each group is presented. (b) Percentages of Vβ expressing CD4⁺CD8⁻, CD4⁻CD8⁺ thymocytes, spleen cells, and lymph node cells. Data shown are ± SEM from an experiment involving 2 or 3 mice per group that was repeated three times.