Figure 2.

DEL and P2 mutant cells have increased Ca²⁺ transients. Shown are representative real time Fura-2 ratios (340:380) for cells activated with 1 μg/ml F(ab′)₂ anti-IgM. (A) Cells expressing the indicated CD22 construct or vector alone were activated by BCR cross-linking and total calcium flux was monitored_. (B) Ca²⁺ release was measured by activating cells in the presence of 2 mM EGTA. Representative of three or more experiments with independently infected cells.