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. 2002 May 6;195(9):1155–1166. doi: 10.1084/jem.20011547

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Localization of peroxynitrite in S. typhimurium-infected macrophages. Infection of RAW267.4 macrophages and mouse peritoneal macrophages (PM) was performed for 12 h as described in the legend to Fig. 5. The sites of peroxynitrite formation were detected by immunostaining with an anti-nitrotyrosine antibody and a Cy3-conjugated secondary antibody. Samples were analyzed by confocal laser-scanning microscopy and representative images for the localization of S. typhimurium expressing GFP (green) and peroxynitrite (red) are shown. Various levels of nitrotyrosine formation were observed for the SPI2 mutant strain. wt, wild-type.