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Induction of Nitrite Production by RAW267.4 Macrophages
| Nitrite
|
||
|---|---|---|
| L-NMMA | Noninfected | Infected |
| μM | μM | μM |
| 0 | 0 | 8.9 ± 0.9 |
| 5 | 0 | 2.4 ± 0.3 |
| 10 | 0 | 0.4 ± 0.6 |
| 100 | 0 | 0.0 ± 0.0 |
RAW267.4 macrophages were infected with wild-type S. typhimurium at an MOI of 10. Noninternalized bacteria were removed by washing followed by killing with gentamicin. After infection L-NMMA was added as indicated and 8 h after infection, nitrite levels in the culture supernatant were assayed by the Griess reaction.
