FIG. 1.

Detection of TAG in P. falciparum-infected erythrocytes. (A) Uninfected red blood cells (RBC; 5 × 10⁷) or RBCs containing 5 × 10⁷ ring-, trophozoite-, or schizont-stage parasites were lysed, and lipids were processed for TLC as described in the material and methods information online (see the text). Migration of standards is indicated. CHOL, cholesterol. (B) RBCs and Percoll-purified schizont-infected red cells (5 × 10⁷ each) were incubated with [³H]oleic acid for 2 h, and lipids were processed for TLC. The plate was scraped, and radioactivity was quantified by scintillation counting (see materials and methods information online). FA, fatty acid.