Figure 6.
The number of splenic virus specific CD8+ T cells is greater in JNK2−/− mice than in either JNK1−/− or JNK+/+ mice during the secondary response. JNK1−/−, JNK2−/−, and JNK+/+ mice were injected intraperitoneally with LCMV ARM 2 × 105 PFU, rechallenged after 60 d with Cl-13 (106 PFU intravenously), and spleens were harvested 4 d later. Spleen cells were stimulated for 5 h in vitro with virus specific peptides GP aa 33–41 or NP aa 396–404, then stained for CD8 and intracellular IFN-γ. The number of virus-specific T cells was calculated from the number of total spleen cells obtained per animal. Data represent average values obtained from three animals per group and are representative of three independent experiments. *P < 0.05 when compared with JNK+/+.