Table II.
Splenocytes from 7 and 8 d after LCMV Inoculation Were Stimulated In Vitro with LCMV-specific Peptides and Cytokine Production Was Assessed by Flow Cytometry
| IFN-γ
|
IL-2
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Percentage of splenic CD8+ T cellsa , b |
Percentage of splenic CD4+ T cellsa , b |
Percentage of splenic CD8+ T cellsa , b |
Percentage of splenic CD4+ T cellsa , b |
||||||
| Group | GP 33–41 | NP 396–404 | GP 61–80 | NP 309–328 | GP 33–41 | NP 396–404 | GP 61–80 | NP 309–328 | |
| JNK1−/− | D7 | 22 ± 1 | 15 ± 1 | 5.0 ± 0.3 | 1.1 ± 0.3 | 1.8 ± 0.3 | 0.6 ± 0.1 | 2.6 ± 1 | 0.3 ± 0.05 |
| D8 | 36 ± 1 | 18 ± 1 | 4.9 ± 0.5 | 1.7 ± 0.2 | |||||
| JNK2−/− | D7 | 26 ± 2 | 18 ± 2 | 7.4 ± 1.3 | 1.5 ± 0.2 | 1.2 ± 0.1 | 0.6 ± 0.1 | 4.5 ± 0.5 | 0.3 ± 0.05 |
| D8 | 53 ± 2c | 29 ± 3c | 11.1 ± 1.7d | 1.8 ± 0.4 | |||||
| JNK+/+ | D7 | 23 ± 1 | 14 ± 1 | 6.1 ± 1.0 | 1.4 ± 0.5 | 1.4 ± 0.1 | 0.3 ± 0.03 | 3.6 ± 0.5 | 1.0 ± 0.4 |
| D8 | 30 ± 2 | 16 ± 1 | 6.3 ± 0.9 | 2.3 ± 0.3 | |||||
D7, day 7; D8, day 8.
a
Average of four mice per group.
b
Percentage of CD8+ or CD4+ positive for cytokine as measured by flow cytometry ± standard error.
c
P < 0.05 compared to JNK+/+.
d
P = 0.05.