Table I.
Cytokine Production by NK1.1+ and NK1.1− CD1d/α-GC Tetramer+ Thymocytes
| IL-4
|
IFN-γ
|
|||
|---|---|---|---|---|
| NK cell subset |
Day 1 | Day 2 | Day 1 | Day 2 |
| U/ml/105 cellsa | ng/ml/105 cells | |||
| NK1.1− | 4,400 ± 500b | 13,000 ± 1,000 | 2.2 ± 0.3 | 15 ± 3 |
| NK1.1+ | 1,160 ± 80c | 2,900 ± 200c | 16 ± 2c | 150 ± 20c |
FACS®-sorted NK1.1+/− CD1d/α-GC+ thymocytes from 4-wk-old C57BL/6 mice were stimulated in anti-CD3–coated microtitre plates. IL-4 and IFN-γ were measured by ELISA from supernatants which were harvested at 24 and at 48 h.
a
100 U/ml is equivalent to 1 ng/ml (BD PharMingen IL-4 standard).
b
Data represents mean ± standard error for 15 individual culture replicates for NK1.1− CD1d/α-GC+ thymocytes and 10 replicates for NK1.1+ CD1d/α-GC+ from three separate experiments.
c
P < 0.05 compared to NK1.1− subset (Mann-Whitney U rank sum test).