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. 2002 Apr 1;195(7):919–929. doi: 10.1084/jem.20011475

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Comparative analysis of surface antigen expression among BM Lin⁻ and SC12 Lin⁻ populations. BM and SC12 cell suspensions were labeled with antibodies recognizing Ly5.2 (donor population) and lineage antigens (CD3, CD19, Mac-1, NK1.1, and TCR-αβ). Results show the Ly5.2⁺ Lin⁻ gated populations, the dotted-lined histogram representing control isotype for anti-IL-7Rα antibody, the thin-lined histograms representing BM and the bold-lined histograms SC12 cells. (A) Forward scatter. (B) Differential expression of cell surface antigens. (C) Selective coexpression of Thy1.1 antigen by SC12 and BM Lin⁻ CD44⁺ and CD44⁻ populations. Fig. 1 shows one of three independent experiments, all yielding similar results.

Figure 1. — Comparative analysis of surface antigen expression among BM Lin⁻ and SC12 Lin⁻ populations. BM and SC12 cell suspensions were labeled with antibodies recognizing Ly5.2 (donor population) and lineage antigens (CD3, CD19, Mac-1, NK1.1, and TCR-αβ). Results show the Ly5.2⁺ Lin⁻ gated populations, the dotted-lined histogram representing control isotype for anti-IL-7Rα antibody, the thin-lined histograms representing BM and the bold-lined histograms SC12 cells. (A) Forward scatter. (B) Differential expression of cell surface antigens. (C) Selective coexpression of Thy1.1 antigen by SC12 and BM Lin⁻ CD44⁺ and CD44⁻ populations. Fig. 1 shows one of three independent experiments, all yielding similar results.