Table II.

Differential Expression of Developmental Genes in Precursor Populations

	BM Lin− cells	SC12 Lin− CD44+ cells	SC12 Lin− CD44− cells
% GATA-2	51.42a	15.60 (5.80)	6.70 (3.20)
% Pax5	1.60 (0.82)	0.19 (0.08)	0.04 (0.03)
% CD3ε	0.23 (0.13)	1.67 (0.55)	96b
% pre-Tα	Not detectedc	0.62 (0.20)	7.60 (3.40)

Lin⁻ cells were sorted in limiting dilution conditions at two or three different cell densities per well (24 wells per density) and tested for GATA-2, Pax5, CD3ε, and pre-Tα mRNA. The frequency estimates were calculated on wells which were scored positive by HPRT according to the Poisson distribution (reference 33) and 95% confidence limits are shown in brackets. When the frequency of gene expression was high, individual cells were sorted.

^aThe frequency of GATA-2 mRNA expressing cells was estimated in 35 individual BM Lin⁻ cells.

^bThe frequency of CD3ε mRNA expressing cells was estimated in 49 individual SC12 Lin⁻ CD44⁻ cells.

^c8 wells containing 10³ cells per well were analyzed to exclude pre-Tα expression.