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. 2002 Apr 1;195(7):869–879. doi: 10.1084/jem.20011712

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Infection of NKT cells by HIV-1. SEB-stimulated CD4⁺ T cells and α-GalCer stimulated NKT cell cultures were described in Fig. 1 legend. Activated NKT cells were further sorted into CD4⁺ and CD4⁻ subsets. At days 7–10 after activation cells were infected with: (A) GFP-encoding R5-tropic, X4-tropic viruses, or VSV-G pseudotyped HIV vector at MOI of 1. After 3 d, cells were stained with anti-CD4-PE, fixed with 2% paraformaldehyde, and GFP expression was analyzed by FACS^®. (B) Cells were also infected with HSA-encoding nef ⁺ R5-tropic viruses at MOI 1 and 0.01. After 3 and 5 d after infection, cells were stained with FITC-conjugated anti–mouse HSA, fixed with 2% paraformaldehyde, and HSA expression was analyzed by FACS^®.

Figure 4. — Infection of NKT cells by HIV-1. SEB-stimulated CD4⁺ T cells and α-GalCer stimulated NKT cell cultures were described in Fig. 1 legend. Activated NKT cells were further sorted into CD4⁺ and CD4⁻ subsets. At days 7–10 after activation cells were infected with: (A) GFP-encoding R5-tropic, X4-tropic viruses, or VSV-G pseudotyped HIV vector at MOI of 1. After 3 d, cells were stained with anti-CD4-PE, fixed with 2% paraformaldehyde, and GFP expression was analyzed by FACS^®. (B) Cells were also infected with HSA-encoding nef ⁺ R5-tropic viruses at MOI 1 and 0.01. After 3 and 5 d after infection, cells were stained with FITC-conjugated anti–mouse HSA, fixed with 2% paraformaldehyde, and HSA expression was analyzed by FACS^®.

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