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. 2002 Mar 18;195(6):771–780. doi: 10.1084/jem.20011140

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — B-1a cells are absent in the blood of asplenic mice. (A) Representative flow cytometric analysis of peripheral blood cells isolated from C57BL/6 and Hox11-null mice. Cells were stained with antibodies against IgM, IgD, CD5, and B220. IgM^dullIgD^pos (left panels, top region) and IgM^posIgD^dull B lymphocytes (left panels, bottom region) were gated and analyzed for B220 versus CD5 expression (right panels). IgM^dullIgD^pos cells are B220^posCD5^neg mature B lymphocytes (top right panels). Gated IgM^posIgD^dull B lymphocytes (bottom right panels) comprise CD5^posB220^dull B-1a cells (top region) and CD5^negB220^pos B-1b and T1 cells (bottom region). The CD5^posB220^pos B-1a population that remains in Hox11-null mice is indicated with an arrow. (B) Representative flow cytometric analysis of cells stained with antibodies against IgM, IgD, CD5, and Mac-1 isolated from blood of the same C57BL/6 and Hox11-null mice analyzed in A. IgM^posIgD^dull B-lymphocytes were gated and analyzed for Mac-1 versus CD5 expression. Gated IgM^posIgD^dull B lymphocytes comprise CD5^posMac-1^pos B-1a cells (indicated by arrow), CD5^negMac-1^pos B-1b, and CD5^negMac-1^neg T1 cells. (C) Relative proportions of the major B cell populations in the peripheral blood of C57BL/6 and Hox11-null mice. Each value is the average of measurements obtained in five mice. For the purpose of measurement, B-1a cells are defined as IgM^posIgD^dullB220^dullCD5^pos, B-1b as IgM^posIgD^dullMac-1^posCD5^neg, and T1 as IgM^posIgD^dullMac-1^negCD5^neg. IgM^posIgD^dull cells constituted 26.5% ± 5.7% of total B cells (IgM^pos and/or IgD^pos) in the blood of C57BL/6 and 39.5% ± 6.5% in Hox11-null mice.

Figure 2. — B-1a cells are absent in the blood of asplenic mice. (A) Representative flow cytometric analysis of peripheral blood cells isolated from C57BL/6 and Hox11-null mice. Cells were stained with antibodies against IgM, IgD, CD5, and B220. IgM^dullIgD^pos (left panels, top region) and IgM^posIgD^dull B lymphocytes (left panels, bottom region) were gated and analyzed for B220 versus CD5 expression (right panels). IgM^dullIgD^pos cells are B220^posCD5^neg mature B lymphocytes (top right panels). Gated IgM^posIgD^dull B lymphocytes (bottom right panels) comprise CD5^posB220^dull B-1a cells (top region) and CD5^negB220^pos B-1b and T1 cells (bottom region). The CD5^posB220^pos B-1a population that remains in Hox11-null mice is indicated with an arrow. (B) Representative flow cytometric analysis of cells stained with antibodies against IgM, IgD, CD5, and Mac-1 isolated from blood of the same C57BL/6 and Hox11-null mice analyzed in A. IgM^posIgD^dull B-lymphocytes were gated and analyzed for Mac-1 versus CD5 expression. Gated IgM^posIgD^dull B lymphocytes comprise CD5^posMac-1^pos B-1a cells (indicated by arrow), CD5^negMac-1^pos B-1b, and CD5^negMac-1^neg T1 cells. (C) Relative proportions of the major B cell populations in the peripheral blood of C57BL/6 and Hox11-null mice. Each value is the average of measurements obtained in five mice. For the purpose of measurement, B-1a cells are defined as IgM^posIgD^dullB220^dullCD5^pos, B-1b as IgM^posIgD^dullMac-1^posCD5^neg, and T1 as IgM^posIgD^dullMac-1^negCD5^neg. IgM^posIgD^dull cells constituted 26.5% ± 5.7% of total B cells (IgM^pos and/or IgD^pos) in the blood of C57BL/6 and 39.5% ± 6.5% in Hox11-null mice.