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. 2002 Mar 18;195(6):771–780. doi: 10.1084/jem.20011140

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — The peritoneal B-1a cell pool is rapidly reduced following splenectomy. (A) Representative FACS^® analysis of peritoneal B cells isolated 10 d after splenectomy or sham operation and gated for IgM^posMac-1^pos cells. A reduction of the CD5^posB220^dull B-1a population can be seen. (B) Time course of reduction in B-1a cells upon splenectomy. C57BL/6 mice were either splenectomized or sham-operated and the frequency of B220^dullCD5^posIgM^posMac-1^pos B-1a cells determined by flow-cytometry at the indicated time points after surgery. At least three splenectomized and two sham-operated mice were examined for each time point, except for sham-operated day 6 where the cell number from only one mouse is shown. (C) Similar time course for B220^posCD5^negIgM^posMac-1^pos B-1b cells.

Figure 4. — The peritoneal B-1a cell pool is rapidly reduced following splenectomy. (A) Representative FACS^® analysis of peritoneal B cells isolated 10 d after splenectomy or sham operation and gated for IgM^posMac-1^pos cells. A reduction of the CD5^posB220^dull B-1a population can be seen. (B) Time course of reduction in B-1a cells upon splenectomy. C57BL/6 mice were either splenectomized or sham-operated and the frequency of B220^dullCD5^posIgM^posMac-1^pos B-1a cells determined by flow-cytometry at the indicated time points after surgery. At least three splenectomized and two sham-operated mice were examined for each time point, except for sham-operated day 6 where the cell number from only one mouse is shown. (C) Similar time course for B220^posCD5^negIgM^posMac-1^pos B-1b cells.