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. 2002 Mar 18;195(6):771–780. doi: 10.1084/jem.20011140

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — Mice lacking B-1a cells but possessing a spleen fail to respond to immunization with streptococcal polysaccharides. (A) Fetal liver (FL) or bone marrow (BM) cells from C57BL/6 mice were injected intraperitoneally into irradiated Rag2-null mice and four weeks later reconstituted mice were analyzed. A representative FACS^® analysis of total ungated peritoneal cells stained for CD5 and B220 from a FL- and a BM-transferred mouse is shown above. The CD5^posB220^dull B-1a cell population is boxed. The CD5^posB220^pos B cells phenotypically identical to residual CD5^pos B cells of Hox11-null mice are indicated by a box and arrow. (B) Representative FACS^® analyses of CD23^neg-gated spleen cells stained for CD21 and IgM from a FL- and a BM-transferred mouse are shown. The normal position of the MZ B cell population (MZ) is boxed. (C) Specific IgM antibodies in sera of transferred mice. Pneumovax^® 23 was injected intraperitoneally 20 d after transfer. Antibodies were measured by ELISA 1 d before and 6 d after Pneumovax^® 23 injection. The optical density (OD₄₀₅) measured with a 1:80 serum dilution is shown. Each data point indicates the relative antibody level of a single mouse. (D) The response to Pneumovax^® 23 of mice transferred with either bone marrow (BM) or dE14.5 fetal liver cells (FL) is shown as mean fold-increase of specific serum IgM as detected by ELISA. The optical density (OD₄₀₅) was measured with a 1:80 serum dilution. For each route of injection, five mice were transferred with bone marrow and three with fetal liver cells.

Figure 6. — Mice lacking B-1a cells but possessing a spleen fail to respond to immunization with streptococcal polysaccharides. (A) Fetal liver (FL) or bone marrow (BM) cells from C57BL/6 mice were injected intraperitoneally into irradiated Rag2-null mice and four weeks later reconstituted mice were analyzed. A representative FACS^® analysis of total ungated peritoneal cells stained for CD5 and B220 from a FL- and a BM-transferred mouse is shown above. The CD5^posB220^dull B-1a cell population is boxed. The CD5^posB220^pos B cells phenotypically identical to residual CD5^pos B cells of Hox11-null mice are indicated by a box and arrow. (B) Representative FACS^® analyses of CD23^neg-gated spleen cells stained for CD21 and IgM from a FL- and a BM-transferred mouse are shown. The normal position of the MZ B cell population (MZ) is boxed. (C) Specific IgM antibodies in sera of transferred mice. Pneumovax^® 23 was injected intraperitoneally 20 d after transfer. Antibodies were measured by ELISA 1 d before and 6 d after Pneumovax^® 23 injection. The optical density (OD₄₀₅) measured with a 1:80 serum dilution is shown. Each data point indicates the relative antibody level of a single mouse. (D) The response to Pneumovax^® 23 of mice transferred with either bone marrow (BM) or dE14.5 fetal liver cells (FL) is shown as mean fold-increase of specific serum IgM as detected by ELISA. The optical density (OD₄₀₅) was measured with a 1:80 serum dilution. For each route of injection, five mice were transferred with bone marrow and three with fetal liver cells.