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. 2002 Mar 18;195(6):683–694. doi: 10.1084/jem.20010898

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Copyright © 2002, The Rockefeller University Press

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Figure 10. — Overexpression of Bcl-2 by B cells decreases passive death in vitro but does not affect DC acquisition of transferred proteins. (A) Purified B cells from C57BL/6 stock mice and from C57BL/6-TgN(22Wehi) transgenic mice, which overexpress Bcl-2 were labeled with CFSE and cultured in complete media for 16–18 h. The cells were then washed and stained with 7-AAD and B220 and analyzed by flow cytometry. Data were gated for B220⁺ cells and 7-AAD was used as a measure of cell death. Data represent the mean of triplicate cultures and error bars represent standard deviation. The data are representative of two independent experiments. (B) Purified B cells were labeled with CFSE and injected intravenously into B10BR (H-2^kk) recipients. 16 h later, mice were killed and the splenocytes were prepared and analyzed by flow cytometry. Data were gated to exclude dead cells and red blood cells. CD11c⁺ and CFSE⁺ were gated and counted. Each point represent a single mouse. The data represent three independent experiments.

Figure 10. — Overexpression of Bcl-2 by B cells decreases passive death in vitro but does not affect DC acquisition of transferred proteins. (A) Purified B cells from C57BL/6 stock mice and from C57BL/6-TgN(22Wehi) transgenic mice, which overexpress Bcl-2 were labeled with CFSE and cultured in complete media for 16–18 h. The cells were then washed and stained with 7-AAD and B220 and analyzed by flow cytometry. Data were gated for B220⁺ cells and 7-AAD was used as a measure of cell death. Data represent the mean of triplicate cultures and error bars represent standard deviation. The data are representative of two independent experiments. (B) Purified B cells were labeled with CFSE and injected intravenously into B10BR (H-2^kk) recipients. 16 h later, mice were killed and the splenocytes were prepared and analyzed by flow cytometry. Data were gated to exclude dead cells and red blood cells. CD11c⁺ and CFSE⁺ were gated and counted. Each point represent a single mouse. The data represent three independent experiments.