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. 2002 Mar 18;195(6):683–694. doi: 10.1084/jem.20010898

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Antigen concentrated by nontransgenic B cells is effectively transferred for presentation to T cells in vivo. HEL was loaded onto nontransgenic B6 B cells (H-2^bb) using an anti-IgM antibody conjugated with HEL to target HEL to the BCR. (A) HEL loading was measured by flow cytometry using the HEL-binding antibody, HyHEL-9. The histograms represent surface HEL on: Ig^HEL transgenic B cells after loading with HEL (HEL), nontransgenic B cells after loading with anti–IgM-HEL conjugate (αIgM-HEL), nontransgenic thymocytes after loading with anti-IgM-HEL conjugate (thymus), and Ig^HEL transgenic B cells after sham loading (Sham). Data were gated for live lymphocytes and for B220⁺ cells. (B) Loaded donor cells were transferred into B10.BR recipients which had been previously seeded with CFSE-labeled 3A9 HEL-specific transgenic T cells. 14 h after transfer, mice were killed and the spleens prepared for flow cytometry. Data were gated for live lymphocytes and either CFSE⁺CD4⁺ responder T cells (HEL-specific) or CFSE⁻CD4⁺ responder T cells (endogenous), and activation was measured using CD69. Each point represents a single mouse. Data are representative of two experiments.

Figure 3. — Antigen concentrated by nontransgenic B cells is effectively transferred for presentation to T cells in vivo. HEL was loaded onto nontransgenic B6 B cells (H-2^bb) using an anti-IgM antibody conjugated with HEL to target HEL to the BCR. (A) HEL loading was measured by flow cytometry using the HEL-binding antibody, HyHEL-9. The histograms represent surface HEL on: Ig^HEL transgenic B cells after loading with HEL (HEL), nontransgenic B cells after loading with anti–IgM-HEL conjugate (αIgM-HEL), nontransgenic thymocytes after loading with anti-IgM-HEL conjugate (thymus), and Ig^HEL transgenic B cells after sham loading (Sham). Data were gated for live lymphocytes and for B220⁺ cells. (B) Loaded donor cells were transferred into B10.BR recipients which had been previously seeded with CFSE-labeled 3A9 HEL-specific transgenic T cells. 14 h after transfer, mice were killed and the spleens prepared for flow cytometry. Data were gated for live lymphocytes and either CFSE⁺CD4⁺ responder T cells (HEL-specific) or CFSE⁻CD4⁺ responder T cells (endogenous), and activation was measured using CD69. Each point represents a single mouse. Data are representative of two experiments.