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. 2002 Mar 18;195(6):683–694. doi: 10.1084/jem.20010898

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Copyright © 2002, The Rockefeller University Press

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Figure 9. — The number of DCs containing transferred proteins increases linearly with the number of transferred donor cells. Varying numbers of CFSE-labeled B6 (H-2^bb) or B10.BR (H-2^kk) splenocytes were adoptively transferred into B10.BR recipients by intravenous injection. 18 h after the transfer, the mice were killed and the spleens were prepared and analyzed by flow cytometry for the presence of CD11c⁺CFSE^med cells. Data were gated using a wide FCS, SSC gate that includes lymphocytes and larger granular cells. Dead cells were eliminated by negative gating using 7-AAD. Each point represents a single mouse. The data is representative of five independent experiments.