FIG. 1.

Overexpression of the CTS1 gene restores growth of calcineurin mutant strains at 37°C. Isogenic wild-type JEC34 (ura5) and calcineurin MCC3 (cna1 ura5) mutant strains were transformed with the rescued suppressor plasmid pCTS1 or the control plasmid pPm8 and grown on synthetic dextrose medium lacking uracil for 3 days at 25 or 37°C in the presence or absence of 1 μg of FK506/ml.