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. 2003 Oct;2(5):1025–1035. doi: 10.1128/EC.2.5.1025-1035.2003

FIG. 6.

FIG. 6.

cts1Δ mutation confers a separation defect. (A) Isogenic wild-type (JEC21), cts1Δ mutant (DSF45), and cts1-570 mutant (DSF22) strains were grown to logarithmic phase at 25°C in YPD, fixed and permeabilized, stained with Alexa Fluor-conjugated WGA, and visualized by fluorescence and DIC optics. (B) Isogenic wild-type (JEC21) and cts1Δ mutant (DSF45) strains were grown to logarithmic phase at 25°C in YPD, washed, and incubated in PBS in the presence or absence of chitinase for 2 h prior to visualization by DIC optics. Magnification, ×1,000.