Skip to main content
. 2003 Oct;2(5):1025–1035. doi: 10.1128/EC.2.5.1025-1035.2003

FIG. 9.

FIG. 9.

The C2 domain of Cts1 is necessary for growth at 37°C and binds phosphatidylinositol-derived phospholipids. (A) Isogenic wild-type (JEC21) and cts1Δ (DSF45) and cnb1Δ (DSF11) mutant strains overexpressing FLAG-Cts1 under the control of the CTS1 promoter in either sense or antisense orientation or Cts1ΔC2-FLAG were serially diluted and grown on YPD medium for 3 days at 37°C in the presence or absence of 1 μg of FK506/ml. (B) Purified FLAG-Cts1 and Cts1ΔC2-FLAG fusion proteins and the extract control were overlaid on the PIP array membrane. Bound protein was detected with a monoclonal anti-FLAG antibody. The input material for each binding assay was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride, and detected with anti-FLAG antibody. At the bottom of panel B is a table showing the relative density of input sample binding to either PI(4)P or PI(5)P expressed as the percent density of each sample relative to the FLAG-Cts1 sample.