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. 2002 Mar 18;195(6):759–770. doi: 10.1084/jem.20011911

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Expression of rae28 in hematopoietic tissues and purified subpopulations of fetal liver and bone marrow cells. PCR-amplified total cDNAs were prepared from total RNAs extracted from the hematopoietic tissues and fractionated cells and were analyzed by Southern blot analysis with a rae28 probe. Note that high rae28 expression was detected in Lin⁻, Sca-1⁺ subpopulations. BM, bone marrow; FL, fetal liver; Whole, whole fetal liver or bone marrow cells; Lin⁻, Sca-1⁺, Lin⁻, Sca-1⁺ subpopulations of fetal liver and bone marrow cells. Expression of β-actin is shown to confirm equal amount of cDNA on the filter.