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. 2002 May 20;195(10):1233–1245. doi: 10.1084/jem.20011930

Figure 2.

Figure 2.

CD11c+ spleen cells, pulsed in vivo with PPV-VLPs-OVA and injected into naive mice, induce a CTL response against OVA257–264 peptide. C57BL/6 mice were intravenously injected with 10 μg PPV-VLPs-OVA (•, ♦, and ▪) or of control PPV-VLPs (○). 90 min later, CD11c+ (○, •), CD11b+ CD11c− (♦), and B220+ (▪) spleen cells were purified and intravenously injected into naive syngeneic mice (1.5 × 105 cells/mice). 7 d later, spleen cells from injected mice were stimulated in vitro with OVA257–264 peptide for 5 d in the presence of irradiated syngeneic cells. The cytotoxic activity of effector cells was measured on 51Cr-labeled EL-4 cells loaded with OVA257–264. In the inset the CTL activity on unloaded 51Cr-labeled EL-4 cells is shown. Data are expressed as the mean ± SEM of the percent lysis from duplicate samples of three mice per group. One representative experiment out of three is represented.