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. 2002 May 20;195(10):1233–1245. doi: 10.1084/jem.20011930

Figure 6.

Figure 6.

In vivo uptake of PPV-VLPs by DC subpopulations. C57BL/6 mice were intravenously injected with 50 μg Alexa 488–PPV-VLPs-OVA. 90 min or 15 h later their CD11c+ spleen cells were purified and stained with PE and APC-coupled mAbs for analysis on a FACScalibur® cytometer. Noninjected mice were used as control. (A) CD11c+ spleen cells from control mice were stained with APC–anti-CD8α and either PE–anti-CD11c (left) or PE–anti-CD4 (right). In this representative experiment, CD11c+ purified spleen cells contained 53% CD4+ CD8α−, 17% CD4− CD8α+, and 20% CD4− CD8α−. Similar values were obtained from injected mice. (B) FL1 (Alexa 488) histograms, gated on regions marked on the right panel in A, corresponding to CD4− CD8α+, CD4+ CD8α−, and CD4− CD8α− CD11c+ cells purified 90 min (thick line histograms) or 15 h (gray histograms) after injection or from control (thin line histograms). (C) Percentage and mean fluorescence intensity of Alexa 488+ cells, corresponding to the histograms depicted in B. One representative experiment out of two is depicted.