Table I.
CD11b+ CD8α1 cells percentage in total CD8α1 DCs |
CD11b+ CD8α1 cells percentage in total CD11b+ DCs |
|||||||
---|---|---|---|---|---|---|---|---|
Rag2+/+
mice |
Rag2−/−
mice |
Rag2+/+
mice |
Rag2−/−
mice |
|||||
Injection of PPV-VLPs |
− | + | − | + | − | + | − | + |
Exp. 1 | 19 | 43 | 5 | 12 | 7 | 15 | 4 | 4 |
Exp. 2 | 30 | 45 | 17 | 28 | 11 | 23 | 9 | 11 |
Exp. 3 | ND | ND | 19 | 22 | ND | ND | 9 | 8 |
C57BL/6 Rag2+/+ or Rag2−/− mice were intravenously injected with 50 μm PPV-VLPs. Noninjected mice from each strain were used as a control. 15 h later, CD11c+ spleen cells were labeled with CD11b and anti-CD8α antibodies and analyzed on a FACScalibur® cytometer. At least 2 × 104 events were analyzed.