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. 2002 May 20;195(10):1303–1316. doi: 10.1084/jem.20011624

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Immunocytochemical analysis of MVs released by melanoma cells. (A) A cluster of human melanoma cells adherent on glass chamber slides, showing a high level of FasL expression (as detected by G247 mAb), polarized on cellular filopodia and interdigitations (arrowheads). (B) Higher magnification of the FasL stainings on the tip of a melanoma cell interdigitation, showing both the unidirectional secretory behavior (arrowhead) and the marked polar concentration (arrow) of the FasL staining. (C) High electronic magnification of a defined field of a melanoma cell where a cluster of FasL-positive vesicles is under degranulation and a group of three isolated FasL-positive vesicles is detectable 8–10 μm away from the cell membrane (arrow). Immunophenotyping of fixed cells was performed using the PAP method; AEC was used as chromogen and Mayer's haematoxylin for the counterstaining. Final original magnifications: (A) 1,000×; (B) 2,500×; and (C) 3,000×.