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. 2002 May 20;195(10):1303–1316. doi: 10.1084/jem.20011624

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — Immunoelectron microscopy analysis of multivesicular bodies, secretory vesicles, and isolated MVs from melanoma cells. (A and B) Immunoelectron microscopy on ultrathin cryosections of human melanoma cells (see Materials and Methods). (A) Note the clear FasL immunolabeling of exosome-like particles (arrows) degranulating in the extracellular environment by a multivesicular body. In B, multiple degranulation sites are shown with FasL immunolabeled exosome-like vesicles (arrows) scattered into the extracellular environment. Arrowheads outline a wide ER vesicle (ER) that appears negative for the FasL immunolabeling. (C–F) Electron microscopy of the 100,000 g pellet immunogold labeled for (C) FasL, (D) CD63, (E) gp100, and (F) Golgi, respectively. The pellet was composed of 100–200 nm MVs, often detected as aggregates, showing abundant FasL, CD63, and gp100 immunolabeling, while they do not stain for Golgi markers (bars, 0.1 μm).