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. 2003 Oct;2(5):1036–1045. doi: 10.1128/EC.2.5.1036-1045.2003

FIG.3.

FIG.3.

Serotype C strains form dikaryon filaments and fused clamps during mating. Staining filaments from V8 pH 7 medium slide matings with calcofluor white and Sytox Green revealed that, as with serotype D (JEC20 Da × JEC21 Dα [Da × Dα]) (A), both serotype C (B4546 Ca × NIH312 Cα [Ca × Cα]) (B) and intervarietal serotype C-serotype D (B4546 Ca × JEC21 Dα [Ca × Dα] and JEC20 Da × NIH312 Cα [Da × Cα]) (C and D, respectively) crosses form dikaryotic filaments with paired nuclei (white arrows) and fused clamp connections (grey arrows). These same structures could also be observed in a crg1Δ bilateral cross (JF109 Ca × JF101 Cα [Ca crg1 × Cα crg1]) (E), supporting the observation that these strains undergo enhanced mating, as opposed to haploid fruiting which produces unfused clamps and uninucleate filaments. Scale bar, 10 μm.