FIG. 5.

Analysis of meiotic progeny of a serotype C cross. The mating mixture of two marked strains—MATα Nat^r (JF65) and MATa G418^r ura5 (JF66)—was plated onto YEPD medium containing both nourseothricin and G418 to select for recombinants. The parental strains and four progeny, in addition to diploid controls, were analyzed by PCR analysis for the segregation of RFLPs in the PLB1 and SOD1 genes and the mating type loci by using primers for the STE12α, STE20a, MFα, and MFa genes. Segregation of the ability to grow in the absence of uracil supplementation (URA5) and the ability to mate with the parental strains B4546 (serotype C and mating type a) and NIH312 (serotype C and mating type α) was also tested.