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. 2002 Mar 4;195(5):593–602. doi: 10.1084/jem.20011500

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Localization of filovirus GPs in lipid rafts. 293T cells were transfected with Marburg GP (A), Ebo-GPwt, or Ebo-GP_C670/672A (B), or a control plasmid, rafts were prepared by ultracentrifugation and GP was detected by immunoblotting. GM1 was detected by blotting with HRP-CTB in the corresponding fractions spotted on a nitrocellulose membrane, as a control for the quality of raft preparation. (C) 48 h after transfection of 293T cells with Ebola GP, a portion of cells were treated for 20 min with 10 mM methyl-β-cyclodextrin (MβCD) and another portion was left untreated. Raft and soluble fractions were prepared and analyzed by immunoblotting for GP (top panel) and for the raft-excluded protein TrfR (bottom panel).

Figure 1. — Localization of filovirus GPs in lipid rafts. 293T cells were transfected with Marburg GP (A), Ebo-GPwt, or Ebo-GP_C670/672A (B), or a control plasmid, rafts were prepared by ultracentrifugation and GP was detected by immunoblotting. GM1 was detected by blotting with HRP-CTB in the corresponding fractions spotted on a nitrocellulose membrane, as a control for the quality of raft preparation. (C) 48 h after transfection of 293T cells with Ebola GP, a portion of cells were treated for 20 min with 10 mM methyl-β-cyclodextrin (MβCD) and another portion was left untreated. Raft and soluble fractions were prepared and analyzed by immunoblotting for GP (top panel) and for the raft-excluded protein TrfR (bottom panel).