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. 2002 Mar 4;195(5):593–602. doi: 10.1084/jem.20011500

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Incorporation of GM1 in released filovirus virions. (A) Ebola virus was immunoprecipitated from supernatant of infected Vero-E6 cells (lane 2), or uninfected cells as control (lane 1), using anti-GP mAb. After irradiation (2 × 10⁶ R), a fraction of immunoprecipitate (IP) was spotted on nitrocellulose membrane and blotted with HRP-conjugated CTB to detect GM1 (bottom panel). Another portion of the IP was analyzed by SDS-PAGE and immunoblotting with anti-GP mAb (top panel). (B) MBGV (1 μg), prepared by ultracentrifugation and inactivated by radiation (10⁷ R), was analyzed for the presence of GM1, TrfR, and GP in a similar fashion. As control, rafts and soluble fractions from untransfected 293T cells were used.

Figure 4. — Incorporation of GM1 in released filovirus virions. (A) Ebola virus was immunoprecipitated from supernatant of infected Vero-E6 cells (lane 2), or uninfected cells as control (lane 1), using anti-GP mAb. After irradiation (2 × 10⁶ R), a fraction of immunoprecipitate (IP) was spotted on nitrocellulose membrane and blotted with HRP-conjugated CTB to detect GM1 (bottom panel). Another portion of the IP was analyzed by SDS-PAGE and immunoblotting with anti-GP mAb (top panel). (B) MBGV (1 μg), prepared by ultracentrifugation and inactivated by radiation (10⁷ R), was analyzed for the presence of GM1, TrfR, and GP in a similar fashion. As control, rafts and soluble fractions from untransfected 293T cells were used.